In this two-part monograph, the author describes modern methods for the rapid column liquid chromatography of high- and medium-molecular-weight compounds of biological origin, i.e. proteins, peptides, enzymes, nucleic acids, poly- and oligonucleotides, poly- and oligosaccharides, complex biopolymers and biooligomers such as viruses, bacteriophages, ribosomes and glycoconjugates, as well as some other compounds such as immunomodulators. The material is contained in two parts: Part A dealing with general chromatographic theory, principles, materials and techniques; and Part B dealing with the separation of individual compound classes and containing a register of chromatographed substances and a full-title bibliography. Not only is this a specialized, detailed treatise on chromatographic techniques, it also gives a broad, balanced review of rapid separation of all known important biopolymers and biooligomers, both simple and complex, and also of some synthetically prepared and pharmaceutically important biooligomers. In addition, it provides an introduction to the application of HPLC to the study of the structure of these substances.

The science of chromatography: lectures PDF

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