This volume aims at providing a selection of the most effective and widely employed protocols used in the Ewing sarcoma field. Chapters are divided into five sections detailing the molecular biology techniques used to analyze Ewing sarcoma including tissue preservation and methods used in morpho-molecular diagnostics and routine immune-histological analysis. Additional wet-lab protocols comprise i n vitro  techniques including re-expression and functional analyses of  target genes together with  in vivo  tools and models such as patient-derived xenografts (PDX) and orthotopic xenografts for metastasis assessment in Ewing sarcoma. A full section on bioinformatic approaches to Ewing sarcoma covering ChIPseq, epigenetic and systems biology analyses finalizes this volume. Written in the highly successful  Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Cutting-edge and clearly written,  Ewing Methods and Protocols  aims to provide methods to further the study of Ewing sarcoma and bring researchers one step forward in the quest to fight this devastating disease.

Ewing Sarcoma: Methods and Protocols (Methods in Molecular Biology) PDF

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